Cemip2 Knockout gl261 Cell Line

Hot Products

对比

Number:

LM02099024754

Product ID： LM02099024754

Market price

Selling Price:　

Quantity

Stock surplus

加入购物车

立即购买

Message advice>

Antibody custom service consulting

隐藏域元素占位

产品描述
细胞复苏
细胞传代
细胞冻存
抗体验证结果

- Commodity name: Cemip2 Knockout gl261 Cell Line
- Commodity ID: LM02099024754
- Gene Symbol: Kiaa1412 Tmem2
- NCBI Gene ID: 83921
- Ensembl ID: ENSMUSG00000024754
- Uniprot ID: Q5FWI3
- 宿主细胞 / 类型: Gl261/mouse glioma cells
- 规格: 1×10^6 cells/ cryovials
- 生长培养基: DMEM+15%FBS+1% double antibody
- 筛选标记: N/A
- 生长特性: Adherent cells, epithelial-like
- 培养条件: 37℃, 5% CO2 incubator, passage 1/2 to 1/4
- 倍增时间: ~24-36 hours
- 参考换液频率: 2~3 times/week
- 支原体检测结果: Negative
- 敲除效率（Sanger测序）: 100%
- 蛋白质组验证结果: N/A
- 抗体货号: Adding
- 目标基因介绍: Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan fragments of approximately 5 kDa (PubMed:28246172, PubMed:37196767, PubMed:37527776). Very specific to hyaluronan; unable to cleave chondroitin sulfate or dermatan sulfate (PubMed:28246172). Plays an essential role in systemic hyaluronan catabolism and turnover, regulating cell adhesion and migration through hyaluronan degradation at focal adhesion sites (PubMed:33647313, PubMed:34624311). Acts as a regulator of angiogenesis and heart morphogenesis by mediating the degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity).
- 细胞开发路径: Stable KO Cell Pool was generated using the CRISPR-RNP method; Sanger sequencing results showed 100% knockout efficiency in the KO Cell Pool
- 应用: Highly efficient gene knockout cell lines (KO Cell Line) are particularly suitable for preliminary functional analysis, the development of complex disease models, precision drug screening, and a wide range of gene discovery research.
Key words:
- Kiaa1412 Tmem2
01. Pre-warm the complete medium in a 37℃ water bath.
02. Thaw the cryovials in a 37℃ water bath for 1-2 minutes.
03. Transfer the cryovials to a biosafety cabinet and wipe the surface with 70% ethanol.
04. Unscrew the cryovials cap and gently transfer the cell suspension to a sterile centrifuge tube containing 9mL of complete medium.
05. Centrifuge at 125g for 5-7 minutes at room temperature and discard the supernatant.
06. Resuspend the cell pellet with 5mL of complete medium and transfer the cell suspension to a T25 culture flask.
07. Transfer the cells to a 37℃, 5% CO2 incubator for culture.
08. Reference passage ratio: passage 1/2 to 1/4, confluent in 2-3 days.
01. When the cell confluence in the culture flask reaches over 80%-90%, the cells can be passaged.
02. Take out the culture medium, PBS, trypsin (0.25% Trypsin_EDTA Gibco 25200-056), etc. from the 4℃ refrigerator, place them in a 37℃ water bath until the temperature is close to 37℃, take them out, spray 75% alcohol on the surface of the bottle, and place them in a biosafety cabinet.

03. Take out the culture flask to be passaged from the incubator, spray the bottle with 75% alcohol, and place it in a biosafety cabinet.
04. To avoid dispersing the cells, rinse the cells with PBS along the upper wall of the culture flask, discard after washing the cells, add 2mL to T25.
05. Add the corresponding volume of trypsin (1.5mL for T75, 0.5mL for T25), and gently shake the bottle to spread the trypsin evenly over the bottom of the cells. The dosage can be increased or decreased appropriately depending on the actual situation. When most of the cells fall off after about 1-2min, add the corresponding volume of complete culture medium to stop digestion, and gently pipet with a 5mL pipette until all the cells fall off.
06. Transfer the cell suspension to a 15mL centrifuge tube, centrifuge the suspension at 300g for 5min, and discard the supernatant.
07. Transfer 5mL of complete culture medium to resuspend the cells, adjust the inoculation ratio as needed, and supplement the complete culture medium in the culture flask, add to 13-15mL for T75, add to 5mL for T25, and add 1% double antibody.
08. After covering the bottle cap and tightening it, gently shake the bottle to mix the cells evenly, and then place it in a 37℃, 5% CO2 incubator.
01. Prepare freezing solution and pre-chill in advance.
02. Ensure that the cells to be cryopreserved meet the cryopreservation requirements. Check the following conditions under a microscope: healthy appearance and morphological characteristics, growth cycle (late logarithmic phase), no signs of contamination or degradation.
03. Digest and centrifuge the cells (refer to the subculture procedure for specific steps)
04. Add freezing solution to resuspend the cells according to the amount of 1mL per tube, mix well and aliquot into freezing tubes.
05. Place the cells in a programmed cooling box and freeze in a -80℃ refrigerator.
06. Subsequently, transfer the cells to a liquid nitrogen tank for long-term storage.
Antibody validation

MDK Knockout LLC1 Cell Line

Hyal2 Knockout gl261 Cell Line

Gene Knockout Kit Information

Gene Knockout Kit Description

86-180-7171-9206

services@elem-bio.com

All products are used for scientific research only and may not be used for medical diagnosis or drug treatment.

Elem Biotech is only sold to units/enterprise entities.