Gene Knock-In
Based on the leading high-throughput gene knock-in technology platform, Elem can construct a drug target reporter cell line within 3 weeks, providing cell products and customized services for drug target confirmation, lead compound screening, drug mechanism (MOA) research and other drug development stages.
High-throughput gene knock-in platform
Granuloman gene knock-in platforms mainly include the following platforms: single-site integration Hambor cell platform, multi-site integration Shotgun cell platform, induction expression platform based on Cumate induction system, point mutation cell line construction platform and in situ Gene tagging stable cell line construction platform. Provide cell products and customized services for drug target confirmation, lead compound screening, drug mechanism (MOA) research and other drug development stages.
High-throughput single-site integration Hambor cell platform
Technical advantages:
1. Combined with pCargo vector, single copy integration of safe harbor site (Hambor) was realized;
2. Prefabricated Harbor mother cell lines with multiple cell backgrounds, which can express target genes in different cells;
3. Compared with Lentivirus and other random integration, all cells have uniform integration sites and copy numbers, more stable genetics, and clearer phenotypes;
4. Efficient screening approach, 2 weeks to get stable expression cell lines;
5. pCargo series vectors with a variety of expression of the original, you can choose to continue to express or induce expression.
High-throughput multi-site integration Shotgun cell platform
Technical advantages:
1. Combined with pLMPB vector to realize the integration of multiple sites in the genome;
2. Efficient integration efficiency, can ensure the target gene in any cell background genome integration;
3. Compared with Lentivirus and other random integration, it is simpler to use, safe and efficient;
4. Efficient screening approach, 2 weeks to get stable high expression cell lines;
5. pLMPB series vectors have a variety of expression elements, which can be selected for continuous expression or induced expression ﹔
6. Reversible removal of the inserted gene from the cell can be performed to confirm that the genotype is derived from the gene of interest.
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