Multi-site integrated Shotgun stable cell line construction

LiMan Bio's Shotgun stable cell line construction system is developed based on the efficient "cut-and-paste" characteristics of the mobile transposon element in vector DNA and the genome. During the transposon integration process, the target gene is cloned into the LiMan Shotgun transposon integration plasmid, and the transposon recognition elements (ITRS) at both ends of the target gene can be recognized, cut, and "pasted" onto specific sites (TTAA) of genomic DNA by the LiMan shotgun transposase.

LiMan Bio uses a highly efficient Shotgun transposon system to develop a series of Shotgun vectors that can be used to integrate target DNA elements (fluorescent proteins, reporter genes, target genes, Cas9, antibodies, and shRNA) into the eukaryotic cell genome to quickly construct stable cell lines, achieving overexpression or inducible regulation of target elements.

Compared with the lentiviral system, LiMan Bio's Shotgun system has the following advantages:

1. Safer and faster development cycle - no virus packaging, purification, or virus transfection required;

2. No size limit for target DNA elements - can integrate target DNA of 10-100Kb into the genome;

3. Multiple target elements can be simultaneously integrated and inserted;

4. Inducible expression - reduces the burden on cell replication, inducing target DNA elements (genes or shRNA) only during experiments;

5. LiMan has pre-prepared multiple reporter genes, antibody expression, and shRNA expression vectors;

6. Efficient transposase removal system - transient transfection of LiMan Shotgun removal enzyme can efficiently remove the inserted element from the genome, restoring wild-type cells.

 

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