Gene Knockout
The CRISPR RNP method is used for gene knockouts from human and mouse cell lines, especially for immune cells such as B cells, T cells and macrophages, and iPS cells and stem cells are difficult to use traditional methods of gene knockouts based on slow viruses and plasmids.
Special reminder: We do not provide gene editing of clinical sample cells from humans and engineered cells into humans!
High-Throughput Gene Knockout Platform
Nobel Prize for CRISPR/Cas9 technology in 2020. With the maturity of gene editing technology, it plays an increasingly important role in many fields, such as drug target discovery and verification, gene function research, gene therapy and so on. The global scientific research field urgently needs to obtain a series of target genes/whole genome knockout cells to accelerate the progress of basic research and pharmaceutical research and development. At present, there are more than a thousand articles in the use of RNP gene knockout method, and increased year by year, the method has been in the alternative slow virus and plasmid method, become the preferred method of high-throughput gene knockout. Elem has an automated, high-throughput gene knockout standardized production platform to achieve a monthly production capacity of 400 + KO cells with high knockout efficiency. Elem's human genome-wide array knockout library has been screened and verified in human primary cells through high connotation screening, and more than 80% of the targets have achieved knockout efficiency of more than 70%.
The high-throughput gene knockout experiment process simplifies your knockout experiment.
Our experimental process aims to make gene knockout experiments simpler and easier than ever before!
Our team can provide a range of key services to support gene editing:
Day 1-2
demand and analysis
Which Cell to Knockout?
Identification of target gene expression in target cells: deep proteomic analysis services
Day 2-6
Project preparation
Can you knock it out?
Necessity analysis of target genes in target cells: Essential score analysis
Day 7
cell infection
How to knock out?
Elem high-throughput gene knockout: RNP method.
Day 10
Knockout Efficiency Analysis
How efficient is the knockout?
Analysis of target gene knockout efficiency: double guarantee of Sanger sequencing and proteomics
The Whole Genome Gene Knockout Array Library of Elem
Evaluation of KO Efficiency of Elem CRISPR Library by High Content Screening
Characteristics of Elem Whole Genome Knockout Array Library
1. Complete human genome coverage, targeting 19,883 genes, including ~ 8800 target genes related to drug development;
2. Design multiple sgRNAs per target gene to ensure high KO efficiency;
3. Use sgRNAs with special chemical modifications to improve stability and block innate immune responses;
4. Designed a full set of sequencing primers for the whole genome through experimentally verified high KO efficiency;
5. Editing efficiency can be determined in 3 days by simple Sanger sequencing.
CRISPR Z’ analysis
Partial Knockout Statistics
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