Gene Knockout
The CRISPR RNP method is used to target gene knockout from human and murine cell lines, which is particularly suitable for immune cells such as B cells, T cells, and macrophages, and iPS cells and stem cells are difficult to use traditional methods such as lentivirus and plasmid methods to present gene knockouts.
Special reminder: We do not provide gene editing of clinical sample cells from the human body and engineered cells from the human body!
Granman focuses on providing cell in vitro gene editing solutions, providing 6 options for in vitro gene knockout for human and mouse cells (immune cells, stem cells, iPS, common cell lines, etc.). You can choose the right product form according to the cycle requirements, quality requirements and budget of your research!
- sgRNAs Synthesis Service: 1~2 weeks delivery, sgRNA sequences need to be provided by customers;
- LM EasyKO Kits (RNP method): 1~2 weeks delivery, containing 2~3 validated sgRNA/targets, no need to provide sgRNA sequences;
- In-stock KO Pools: In-stock KO polyclonal with delivery in 2~3 weeks, gene-level verification available (recommended product!) ；
- Custom KO Pools Service: Customized KO polyclonal, delivered in 4~5 weeks, providing gene-level verification;
- Custom KO Clone Service: 6~7 weeks delivery, 100% protein level validated KO cell line (recommended service!) ；
- Custom Services: 8~10 weeks delivery, providing solutions for different types of knockouts/knockdowns, gene knockouts for special needs (non-coding regions, large fragment knockouts, specified region fuzzy/precision knockouts, double/triple knockouts, etc.) and knockdown protocols for essential genes (inducible shRNA knockdown stable cell lines).
High-Throughput Gene Knockout Platform
Nobel Prize for CRISPR/Cas9 technology in 2020. With the maturity of gene editing technology, it plays an increasingly important role in many fields, such as drug target discovery and verification, gene function research, gene therapy and so on. The global scientific research field urgently needs to obtain a series of target genes/whole genome knockout cells to accelerate the progress of basic research and pharmaceutical research and development. At present, there are more than a thousand articles in the use of RNP gene knockout method, and increased year by year, the method has been in the alternative slow virus and plasmid method, become the preferred method of high-throughput gene knockout. Elem has an automated, high-throughput gene knockout standardized production platform to achieve a monthly production capacity of 400 + KO cells with high knockout efficiency. Elem's human genome-wide array knockout library has been screened and verified in human primary cells through high connotation screening, and more than 80% of the targets have achieved knockout efficiency of more than 70%.
The high-throughput gene knockout experiment process simplifies your knockout experiment.
Our experimental process aims to make gene knockout experiments simpler and easier than ever before!
Our team can provide a range of key services to support gene editing:
Day 1-2
demand and analysis
Which Cell to Knockout?
Identification of target gene expression in target cells: deep proteomic analysis services
Day 2-6
Project preparation
Can you knock it out?
Necessity analysis of target genes in target cells: Essential score analysis
Day 7
cell infection
How to knock out?
Elem high-throughput gene knockout: RNP method.
Day 10
Knockout Efficiency Analysis
How efficient is the knockout?
Analysis of target gene knockout efficiency: double guarantee of Sanger sequencing and proteomics
The Granman team provides in vitro gene knockout solutions
Evaluation of KO Efficiency of Elem CRISPR Library by High Content Screening
Advantages of granulomant whole-genome knockout array libraries
1. Complete human genome coverage, targeting 19,883 genes, including ~8800 target genes related to drug development;
2. Multiple sgRNAs are designed for each target gene to ensure high KO efficiency;
3. Use of specially chemically modified sgRNAs to improve stability and block innate immune responses;
4. High KO efficiency verified by experiments, a full set of sequencing primers for the whole genome was designed;
5. With simple Sanger sequencing, editing efficiency can be determined in 3 days.
CRISPR Z’ analysis
Partial Knockout Statistics
Copyright © Elem Biotechnology (Wuhan) Co., Ltd.
LM