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LM EasyKO Control Kit (RNP Method)

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LM04277734-REF

Product ID： LM04277734-REF

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Selling Price:　 1499

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  • 产品描述
    • Commodity name: LM EasyKO Control Kit (RNP Method)
    • Commodity ID: LM04277734-REF
    • Gene Symbol: TRAC TCRA
    • NCBI Gene ID: 28755
    • Ensembl ID: ENSG00000277734
    • Uniprot ID: P01848
    • 物种: Human-derived
    • 规格: 3 reactions/1 target gene, 1 target gene/1.5 mL centrifuge tube
    • 储存条件: -80°C for 1 year, -20°C for 6 months; avoid repeated freeze-thaw cycles.
    • 试剂组成: Chemically synthesized sgRNA (90 pmol) 3 primers (100 pmol each) LM cell lysis buffer (100 µL) Cas9 protein (3 µg) Transfection reagent (20 µL)
    • 产品优势: 1. From gene editing to knockout validation—all in one kit, with zero prior knowledge required for gene knockout. 2. Significantly saves time. For human‑derived genes, there’s no need to design sgRNAs from scratch or verify their functionality; the kit includes sgRNA sequences that have been rigorously validated by extensive experimental data. Additionally, PCR and sequencing primers for target genes are not required. 3. Highly cost‑effective: the total cost of all reagents needed for a single gene knockout is under RMB 450, whereas even bulk‑packaged reagents—such as transfection reagents, lentiviral plasmids, cell lysis buffers, and PCR polymerases—far exceed this price. 4. Enables rapid initiation of gene knockout experiments, streamlining procurement, experimentation, and pre‑ and post‑sales support. By employing the CRISPR‑RNP knockout method, which is widely recognized in high‑impact international publications as superior, you can obtain cleaner, more reliable knockout cell lines.
    • 应用: This kit is designed for in vitro gene knockout experiments in human eukaryotic cells. It employs multiple chemically synthesized, modified single‑strand guide RNAs (sgRNAs) to target the desired gene and utilizes ribonucleoprotein complexes (Cas9–sgRNA RNPs) to mediate gene disruption. Following transfection of the RNPs into cells, cleavage occurs at the early exons of the target gene, leading to exon loss or frameshift mutations and enabling efficient, rapid gene knockout.

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Classification: LM EasyKO Gene Knockout Kit (RNP Method)

Gene Knockout Kit Information

Gene Symbol

TRAC TCRA

NCBI Gene ID

28755

Ensembl ID

ENSG00000277734

Uniprot ID

P01848

Specifications

3 reactions/1 target gene, 1 target gene/1.5 mL centrifuge tube

Species

Human-derived

Storage conditions

-80°C for 1 year, -20°C for 6 months; avoid repeated freeze-thaw cycles.

Reagent composition

Chemically synthesized sgRNA (90 pmol) 3 primers (100 pmol each) LM cell lysis buffer (100 µL) Cas9 protein (3 µg) Transfection reagent (20 µL)

Gene Knockout Kit Description

Product advantages

1. From gene editing to knockout validation—all in one kit, with zero prior knowledge required for gene knockout. 2. Significantly saves time. For human‑derived genes, there’s no need to design sgRNAs from scratch or verify their functionality; the kit includes sgRNA sequences that have been rigorously validated by extensive experimental data. Additionally, PCR and sequencing primers for target genes are not required. 3. Highly cost‑effective: the total cost of all reagents needed for a single gene knockout is under RMB 450, whereas even bulk‑packaged reagents—such as transfection reagents, lentiviral plasmids, cell lysis buffers, and PCR polymerases—far exceed this price. 4. Enables rapid initiation of gene knockout experiments, streamlining procurement, experimentation, and pre‑ and post‑sales support. By employing the CRISPR‑RNP knockout method, which is widely recognized in high‑impact international publications as superior, you can obtain cleaner, more reliable knockout cell lines.

Application

This kit is designed for in vitro gene knockout experiments in human eukaryotic cells. It employs multiple chemically synthesized, modified single‑strand guide RNAs (sgRNAs) to target the desired gene and utilizes ribonucleoprotein complexes (Cas9–sgRNA RNPs) to mediate gene disruption. Following transfection of the RNPs into cells, cleavage occurs at the early exons of the target gene, leading to exon loss or frameshift mutations and enabling efficient, rapid gene knockout.