ITPKA CRISPR Easy KO Kit (Enhanced Version)
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Product ID： LM04137825-PRO
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- 产品描述
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- Brand: ELEM粒曼
- Commodity name: ITPKA CRISPR Easy KO Kit (Enhanced Version)
- Commodity ID: LM04137825-PRO
- Gene Symbol: ITPKA
- NCBI Gene ID: 3706
- Ensembl ID: ENSG00000137825
- Uniprot ID: P23677
- 物种: Human-derived
- 规格: 5–10 reactions per target gene, 1 target gene per 1.5 mL centrifuge tube
- 储存条件: -80°C for 1 year, -20°C for 6 months; avoid repeated freeze-thaw cycles.
- 试剂组成: Chemically synthesized sgRNAs (2–3 sequences), 200 pmol each Three primers (PCR forward and reverse primers, plus a sequencing primer), 500 pmol each LM cell lysis buffer (500 µL) Cas9 protein (12 µg) LM RNP‑specific transfection reagent (50 µL)
- 产品优势: 1. From gene editing to knockout validation—all in one kit, with zero prior knowledge required for gene knockouts. 2. Significantly saves time. For human‑derived genes, you don’t need to design sgRNAs from scratch or verify their functionality; the kit includes sgRNA sequences that have been rigorously validated by extensive experimental data. Additionally, there’s no need to validate PCR primers or sequencing primers for your target gene. 3. Highly cost‑effective: the total cost of all reagents needed for a single gene knockout is under RMB 450, whereas even bulk‑packaged reagent sets—such as transfection reagents, lentiviral plasmids, cell lysis buffers, or PCR polymerases—far exceed this price. 4. Enables rapid and straightforward initiation of gene knockout experiments, saving you time and effort across procurement, experimentation, and pre‑ and post‑sales support. By employing the CRISPR RNP knockout method—widely recognized in high‑impact international publications as superior—you can obtain cleaner, more reliable knockout cell lines.
- 应用: This kit is designed for in vitro gene knockout experiments in human eukaryotic cells. It employs multiple chemically synthesized, modified single‑strand guide RNAs (sgRNAs) to target the desired gene and utilizes ribonucleoprotein complexes (Cas9–sgRNA RNPs) to mediate gene disruption. Following transfection of the RNPs into cells, cleavage occurs at the early exons of the target gene, leading to exon loss or frameshift mutations and enabling efficient, rapid gene knockout.
The LIMAN Bio LM EasyKO Gene Knockout Kit (RNP Method) includes all the reagents required for knocking out target genes using the CRISPR/Cas9 RNP approach, along with sequencing primers for verifying knockout efficiency. With this kit, you can achieve over 70% knockout efficiency in cell lines, primary cells, immune cells, and iPSCs in as little as three days. In addition, when used with LIMAN’s dedicated CRISPR electroporator, the kit enables highly efficient genome editing even in challenging cell types, such as stem cells, iPSCs, immune cells, and primary cells.
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Classification: LM EasyKO Gene Knockout Kit (RNP Method)
Gene Knockout Kit Information
Gene Symbol
ITPKA
NCBI Gene ID
3706
Ensembl ID
ENSG00000137825
Uniprot ID
P23677
Specifications
5–10 reactions per target gene, 1 target gene per 1.5 mL centrifuge tube
Species
Human-derived
Storage conditions
-80°C for 1 year, -20°C for 6 months; avoid repeated freeze-thaw cycles.
Reagent composition
Chemically synthesized sgRNAs (2–3 sequences), 200 pmol each
Three primers (PCR forward and reverse primers, plus a sequencing primer), 500 pmol each
LM cell lysis buffer (500 µL)
Cas9 protein (12 µg)
LM RNP‑specific transfection reagent (50 µL)
Gene Knockout Kit Description
Product advantages
1. From gene editing to knockout validation—all in one kit, with zero prior knowledge required for gene knockouts.
2. Significantly saves time. For human‑derived genes, you don’t need to design sgRNAs from scratch or verify their functionality; the kit includes sgRNA sequences that have been rigorously validated by extensive experimental data. Additionally, there’s no need to validate PCR primers or sequencing primers for your target gene.
3. Highly cost‑effective: the total cost of all reagents needed for a single gene knockout is under RMB 450, whereas even bulk‑packaged reagent sets—such as transfection reagents, lentiviral plasmids, cell lysis buffers, or PCR polymerases—far exceed this price.
4. Enables rapid and straightforward initiation of gene knockout experiments, saving you time and effort across procurement, experimentation, and pre‑ and post‑sales support. By employing the CRISPR RNP knockout method—widely recognized in high‑impact international publications as superior—you can obtain cleaner, more reliable knockout cell lines.
Application
This kit is designed for in vitro gene knockout experiments in human eukaryotic cells. It employs multiple chemically synthesized, modified single‑strand guide RNAs (sgRNAs) to target the desired gene and utilizes ribonucleoprotein complexes (Cas9–sgRNA RNPs) to mediate gene disruption. Following transfection of the RNPs into cells, cleavage occurs at the early exons of the target gene, leading to exon loss or frameshift mutations and enabling efficient, rapid gene knockout.
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