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SLC16A1 CRISPR Easy KO Kit (Enhanced Version)

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LM04155380-PRO

Product ID： LM04155380-PRO

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Selling Price:　 3680

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  • 产品描述
    • Brand: ELEM粒曼
    • Commodity name: SLC16A1 CRISPR Easy KO Kit (Enhanced Version)
    • Commodity ID: LM04155380-PRO
    • Gene Symbol: SLC16A1 MCT1
    • NCBI Gene ID: 6566
    • Ensembl ID: ENSG00000155380
    • Uniprot ID: P53985
    • 物种: Human-derived
    • 规格: 5–10 reactions per target gene, 1 target gene per 1.5 mL centrifuge tube
    • 储存条件: -80°C for 1 year, -20°C for 6 months; avoid repeated freeze-thaw cycles.
    • 试剂组成: Chemically synthesized SgRNAs (2–3 sequences), 200 pmol each Three primers (PCR forward and reverse primers, plus a sequencing primer), 500 pmol each LM cell lysis buffer (500 µL) Cas9 protein (12 µg) LM RNP‑specific transfection reagent (50 µL)
    • 产品优势: 1. From gene editing to knockout validation—all in one kit, with zero prior knowledge required for gene knockout. 2. Significantly saves time. For human‑derived genes, there’s no need to design sgRNAs from scratch or verify their functionality; the kit includes sgRNA sequences that have been rigorously validated by extensive experimental data. Additionally, PCR and sequencing primers for target genes are unnecessary. 3. Highly cost‑effective: the total cost of all reagents needed for a single gene knockout is under RMB 450, whereas even bulk‑packaged reagents—such as transfection reagents, lentiviral plasmids, cell lysis buffers, and PCR polymerases—far exceed this price. 4. Enables rapid initiation of gene‑knockout experiments, streamlining procurement, experimentation, and pre‑ and post‑sales support. By employing the CRISPR RNP‑mediated knockout method, which is widely recognized in high‑impact international publications as superior, you can obtain cleaner, more reliable knockout cell lines.
    • 应用: This kit is designed for in vitro gene knockout experiments in human eukaryotic cells. It employs multiple chemically synthesized, modified single‑strand guide RNAs (sgRNAs) to target the desired gene and utilizes ribonucleoprotein complexes (Cas9–sgRNA RNPs) to mediate gene disruption. Following transfection of the RNPs into cells, cleavage occurs at the early exons of the target gene, leading to fragment loss or frameshift mutations and enabling efficient, rapid gene knockout.
    The LIMAN Bio LM EasyKO Gene Knockout Kit (RNP Method) includes all the reagents required for knocking out target genes using the CRISPR/Cas9 RNP approach, along with sequencing primers for verifying knockout efficiency. With this kit, you can achieve over 70% knockout efficiency in cell lines, primary cells, immune cells, and iPSCs in as little as three days. In addition, when used with LIMAN’s dedicated CRISPR electroporator, it enables highly efficient genome editing even in challenging cell types, such as stem cells, iPSCs, immune cells, and primary cells.

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Classification: LM EasyKO Gene Knockout Kit (RNP Method)

Gene Knockout Kit Information

Gene Symbol

SLC16A1 MCT1

NCBI Gene ID

6566

Ensembl ID

ENSG00000155380

Uniprot ID

P53985

Specifications

5–10 reactions per target gene, 1 target gene per 1.5 mL centrifuge tube

Species

Human-derived

Storage conditions

-80°C for 1 year, -20°C for 6 months; avoid repeated freeze-thaw cycles.

Reagent composition

Chemically synthesized SgRNAs (2–3 sequences), 200 pmol each Three primers (PCR forward and reverse primers, plus a sequencing primer), 500 pmol each LM cell lysis buffer (500 µL) Cas9 protein (12 µg) LM RNP‑specific transfection reagent (50 µL)

Gene Knockout Kit Description

Product advantages

1. From gene editing to knockout validation—all in one kit, with zero prior knowledge required for gene knockout. 2. Significantly saves time. For human‑derived genes, there’s no need to design sgRNAs from scratch or verify their functionality; the kit includes sgRNA sequences that have been rigorously validated by extensive experimental data. Additionally, PCR and sequencing primers for target genes are unnecessary. 3. Highly cost‑effective: the total cost of all reagents needed for a single gene knockout is under RMB 450, whereas even bulk‑packaged reagents—such as transfection reagents, lentiviral plasmids, cell lysis buffers, and PCR polymerases—far exceed this price. 4. Enables rapid initiation of gene‑knockout experiments, streamlining procurement, experimentation, and pre‑ and post‑sales support. By employing the CRISPR RNP‑mediated knockout method, which is widely recognized in high‑impact international publications as superior, you can obtain cleaner, more reliable knockout cell lines.

Application

This kit is designed for in vitro gene knockout experiments in human eukaryotic cells. It employs multiple chemically synthesized, modified single‑strand guide RNAs (sgRNAs) to target the desired gene and utilizes ribonucleoprotein complexes (Cas9–sgRNA RNPs) to mediate gene disruption. Following transfection of the RNPs into cells, cleavage occurs at the early exons of the target gene, leading to fragment loss or frameshift mutations and enabling efficient, rapid gene knockout.