Ces3b CRISPR Easy KO Kit (Mus) (Enhanced Version)
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Product ID： LM04062181-PRO
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- 产品描述
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- Brand: ELEM粒曼
- Commodity name: Ces3b CRISPR Easy KO Kit (Mus) (Enhanced Version)
- Commodity ID: LM04062181-PRO
- Gene Symbol: Ces3b Gm4738
- NCBI Gene ID: 13909
- Ensembl ID: ENSMUSG00000062181
- Uniprot ID: Q8VCU1
- 物种: Mouse-derived
- 规格: 5–10 reactions per target gene, 1 target gene per 1.5 mL centrifuge tube
- 储存条件: -80℃ for 1 year, -20℃ for 6 months; avoid repeated freeze-thaw cycles.
- 试剂组成: Chemically synthesized SgRNAs (2–3 sequences), 200 pmol each Three primers (PCR forward and reverse primers, plus a sequencing primer), 500 pmol each LM cell lysis buffer (500 µL) Cas9 protein (12 µg) LM RNP‑based transfection reagent (50 µL)
- 产品优势: 1. From gene editing to knockout validation—all in one kit, with zero prior requirements for gene knockout. 2. Significantly saves time. For mouse genes, you don’t need to design sgRNAs from scratch or verify their functionality. The sgRNA sequences provided in this kit have been rigorously validated by extensive experimental data; there’s no need to validate PCR primers or sequencing primers for the target gene either. 3. Highly cost-effective: the total cost of all reagents required for a single gene knockout is under RMB 450 on average, whereas even bulk‑packaged reagent products—such as transfection reagents, lentiviral plasmids, cell lysis buffers, and PCR polymerases—far exceed this price. 4. Gene knockout experiments are quick and easy to get started with, saving you time and effort throughout procurement, experimentation, and pre‑ and post‑sales support. By employing the CRISPR RNP knockout method—widely recognized in high‑impact international publications as superior—you can obtain cleaner, more reliable knockout cell lines.
- 应用: This kit is designed for in vitro gene knockout experiments in mouse-derived eukaryotic cells. It employs multiple chemically synthesized, modified single‑strand guide RNAs (sgRNAs) to target the desired gene and utilizes ribonucleoprotein complexes (Cas9–gRNA ribonucleoproteins, RNPs) to mediate gene disruption. Following transfection of the RNPs into cells, cleavage occurs at the early exons of the target gene, leading to fragment loss or frameshift mutations and enabling efficient, rapid gene knockout.
The LM EasyKO Gene Knockout Kit (RNP Method) from LIMAN Biotech includes all the reagents required for knocking out target genes using the CRISPR/Cas9 RNP approach, along with sequencing primers for verifying knockout efficiency. With this kit, you can achieve over 70% knockout efficiency in cell lines, primary cells, immune cells, and iPSCs in as little as three days. In addition, when used with LIMAN’s proprietary CRISPR electroporator, it enables highly efficient genome editing even in challenging cell types, such as stem cells, iPSCs, immune cells, and primary cells.
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Classification: LM EasyKO Gene Knockout Kit (RNP Method)
Gene Knockout Kit Information
Gene Symbol
Ces3b Gm4738
NCBI Gene ID
13909
Ensembl ID
ENSMUSG00000062181
Uniprot ID
Q8VCU1
Specifications
5–10 reactions per target gene, 1 target gene per 1.5 mL centrifuge tube
Species
Mouse-derived
Storage conditions
-80℃ for 1 year, -20℃ for 6 months; avoid repeated freeze-thaw cycles.
Reagent composition
Chemically synthesized SgRNAs (2–3 sequences), 200 pmol each
Three primers (PCR forward and reverse primers, plus a sequencing primer), 500 pmol each
LM cell lysis buffer (500 µL)
Cas9 protein (12 µg)
LM RNP‑based transfection reagent (50 µL)
Gene Knockout Kit Description
Product advantages
1. From gene editing to knockout validation—all in one kit, with zero prior requirements for gene knockout.
2. Significantly saves time. For mouse genes, you don’t need to design sgRNAs from scratch or verify their functionality. The sgRNA sequences provided in this kit have been rigorously validated by extensive experimental data; there’s no need to validate PCR primers or sequencing primers for the target gene either.
3. Highly cost-effective: the total cost of all reagents required for a single gene knockout is under RMB 450 on average, whereas even bulk‑packaged reagent products—such as transfection reagents, lentiviral plasmids, cell lysis buffers, and PCR polymerases—far exceed this price.
4. Gene knockout experiments are quick and easy to get started with, saving you time and effort throughout procurement, experimentation, and pre‑ and post‑sales support. By employing the CRISPR RNP knockout method—widely recognized in high‑impact international publications as superior—you can obtain cleaner, more reliable knockout cell lines.
Application
This kit is designed for in vitro gene knockout experiments in mouse-derived eukaryotic cells. It employs multiple chemically synthesized, modified single‑strand guide RNAs (sgRNAs) to target the desired gene and utilizes ribonucleoprotein complexes (Cas9–gRNA ribonucleoproteins, RNPs) to mediate gene disruption. Following transfection of the RNPs into cells, cleavage occurs at the early exons of the target gene, leading to fragment loss or frameshift mutations and enabling efficient, rapid gene knockout.
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