KIF4A Knockout 786-O Cell Line

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LM02600090889

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产品描述
细胞复苏
细胞传代
细胞冻存
抗体验证结果

- Brand: ELEM粒曼
- Commodity name: KIF4A Knockout 786-O Cell Line
- Commodity ID: LM02600090889
- Gene Symbol: KIF4A KIF4
- Ensembl ID: ENSG00000090889
- Uniprot ID: O95239
- 宿主细胞 / 类型: 786-O/human renal clear cell adenocarcinoma cells
- NCBI Gene ID: 24137
- 规格: 1×10^6 cells/frozen vial
- 生长培养基: RPMI-1640 + 10% FBS + 1% P,S
- 筛选标记: N/A
- 生长特性: Adherent cells, epithelial-like
- 培养条件: Incubator at 37°C with 5% CO2; passage every 1/3 to 1/4 of the way through the culture.
- 倍增时间: ~24-36 hours
- 参考换液频率: 2–3 times per week
- 支原体检测结果: Negative
- 敲除效率（Sanger测序）: 100%
- 蛋白质组验证结果: N/A
- 抗体货号: Adding...
- 目标基因介绍: Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis (PubMed:29848660). Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis (PubMed:15297875, PubMed:15625105). May play a role in mitotic chromosomal positioning and bipolar spindle stabilization (By similarity).
- 细胞开发路径: A stable KO cell line was generated using the CRISPR‑RNP approach; Sanger sequencing confirmed 100% knockout efficiency in the KO cell line.
- 应用: A gene knockout cell line with high knockout efficiency, particularly well suited for preliminary functional analyses, the development of complex disease models, precision drug screening, and broad‑scale gene discovery studies.
Key words:
- KIF4A KIF4
01. Preheat the complete culture medium in a 37°C water bath.
02. Thaw the cryovial in a 37°C water bath for 1–2 minutes.
03. Transfer the cryovial to a biosafety cabinet and wipe its surface with 70% ethanol.
04. Unscrew the cap of the cryovial and gently transfer the cell suspension into a sterile centrifuge tube containing 9 mL of complete culture medium.
05. Centrifuge at room temperature at 125g for 5–7 minutes, then discard the supernatant.
06. Resuspend the cell pellet in 5 mL of complete culture medium, and transfer the cell suspension to a T25 culture flask.
07. Transfer the cells to a 37°C incubator with 5% CO2 for culture.
08. Recommended passage ratio: 1/3 to 1/4; cells will confluence in 2–3 days.
01. When the cells in the culture flask reach a confluence of 80%–90% or higher, cell passage can be performed.
02. Remove the culture medium, PBS, and trypsin (0.25% Trypsin‑EDTA, Gibco 25200‑056) from the 4°C refrigerator, place them in a 37°C water bath, and once their temperature approaches 37°C, take them out. Spray the exterior of the bottles with 75% ethanol, then transfer them to a biosafety cabinet.

03. Remove the culture flask to be passaged from the incubator, spray the exterior of the flask with 75% ethanol, and place it inside a biosafety cabinet.
04. To avoid dispersing the cells, gently rinse the cells along the inner wall of the culture flask with PBS; discard the wash solution, and add 2 mL of PBS to the T25 flask.
05. Add the appropriate volume of trypsin (1.5 mL for a T75 flask, 0.5 mL for a T25 flask), and gently swirl the flask to ensure the enzyme evenly covers the cell‑covered bottom. Adjust the volume as needed based on the specific situation. After approximately 1–2 minutes, when most of the cells have detached, add the corresponding volume of complete culture medium to stop the digestion, then gently pipette up and down with a 5‑mL pipette until all cells are fully detached.
06. Transfer the cell suspension to a 15 mL centrifuge tube, centrifuge at 300 g for 5 minutes, and discard the supernatant.
07. Transfer 5 mL of complete culture medium to resuspend the cells, adjust the seeding ratio as needed, and replenish the culture flask with complete medium—add up to 13–15 mL for a T75 flask and 5 mL for a T25 flask—then supplement with 1% double antibiotic.
08. After capping the bottle and tightening the cap, gently shake the vial to ensure uniform mixing of the cells, then place it in a 37°C incubator with 5% CO2.
01. Prepare the cryopreservation solution and pre-cool it in advance.
02. Ensure that the cells to be cryopreserved meet the cryopreservation requirements, and verify the following conditions under a microscope: healthy appearance and morphological characteristics, being in the late logarithmic growth phase, and showing no signs of contamination or senescence.
03. Digest and centrifuge the cells (refer to the subculture protocol for specific steps).
04. Resuspend the cells in cryopreservation medium at 1 mL per tube, gently pipette up and down to ensure uniformity, then aliquot into cryovials.
05. Place the cells in a programmed cooling box and freeze them in a −80°C freezer.
06. Subsequently, the cells are transferred to a liquid nitrogen tank for long-term storage.
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