Inducible shRNA knockdown cell line construction service

The Elem organism Cumate induction system is based on the fact that CymR regulatory elements can bind with high affinity and effectively inhibit CuO transcriptional regulatory elements. In the presence of non-toxic Cumate small molecules, the expression of the DNA of interest was suppressed and maintained at very low expression levels. In the presence of Cumate, it can induce the expression of the target DNA in a dose-dependent manner. The Elem Cumate-induced expression system has an excellent signal-to-noise ratio and can be used as a regulatory switch to achieve the target DNA original (egg. White, Cas9,shRNA, etc.) precise regulation of expression.

Elem CreaturescumateThe system can also be usedshRNAThe induction of expression to achieve the knockdown of the protein regulated by the inducer. Utilizing the high efficiency of Elem organismsshRNAThe design tool combined with the use of inducible promoter vector can quickly and efficiently establish inducible target protein knockdown cell lines. with traditionSiRNAIt has a more stable phenotype and excellent reproducibility.

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