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Deubiquitinating Enzymes Library

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LM06‐DUB

Product ID: LM06‐DUB

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Classification: Gene-editing library

Product Details Introduction

I. Product Overview

The Deubiquitinating Enzymes Library comprises 117 genes encoding deubiquitinating enzymes (DUBs), comprehensively covering major DUB families such as USPs (Ubiquitin-specific Proteases), UCHs (Ubiquitin C-terminal Hydrolases), OTUs, and MJD/JAMMs. By modulating protein ubiquitination levels, deubiquitinating enzymes play critical roles in regulating protein stability, signal transduction, DNA damage repair, cell-cycle control, immune responses, and tumorigenesis and tumor progression.

 

II. Application Scenarios

This library is suitable for research on protein degradation pathways, studies of PROTAC mechanisms, screening for the regulation of target protein stability, identification of drug targets, and analysis of ubiquitination regulatory networks.

 

III. Core Advantages

  • Precise Coverage: By selecting core targets within specific biological pathways, we maximize the success rate of high-throughput screening (HTS).
  • Data completeness: Each shipment is accompanied by a complete plate map and detailed files containing gene/compound information for each well (in Excel or PDF format).
  • Flexible Customization: The aliquot format can be tailored to meet specific experimental requirements, such as 96- or 384-well plates and liquid or lyophilized powder forms.
  • Rigorous Quality Control: All components meet stringent industry‑standard quality criteria, such as NGS–based depth of coverage or LC/MS‑based purity verification, ensuring experimental reproducibility.

IV. Specifications and Parameters

KO Lentiviral Library CRISPRi Lentiviral Library Pool KO Lentiviral Pool KO sgRNA library

96-well / 384-well sealable microplates,

With a unique matrix barcode

1 mL cryovial 1 mL cryovial

96-well / 384-well sealable microplates,

With a unique matrix barcode

Titer per well is greater than 2E6/ml. Titer of 1E8/ml or higher Titer of 1E8/ml or higher LC-MS purity analysis of chemically synthesized sgRNAs shows >90% purity.
50 pmol/target (mixture of 2 sgRNAs)
Store at −80°C long-term; avoid repeated freeze-thaw cycles.
Utilize dry ice–based cold packs for end-to-end low-temperature cold-chain transportation.

 

For product details and customized services, please contact Liman Bio!